Citation: Ai Xia HAN, Li Hong NIU, Rui CHANG, Fu Shi ZHANG. Application of Mn (Ⅱ) as a Mimetic Enzyme of Horseradish Peroxidase[J]. Chinese Chemical Letters, ;2005, 16(1): 93-96. shu

Application of Mn (Ⅱ) as a Mimetic Enzyme of Horseradish Peroxidase

1.
Key Lab of Organic Optoelecronics & Molecular Engineering of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084;
2.
Chemical Engineering College of Qinghai University, Xining 810016;
3.
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084

Received Date: 20 November 2003

Fund Project: This work was supported by The National Natural Science Foundation of China (20333080)

In this study, Mn (Ⅱ) as a mimetic enzyme of horseradish peroxidase (HRP) was applied to the determination of hydrogen peroxide (H₂O₂). The method introduced in this paper is based on Mn (Ⅱ)'s catalytic effect on the oxidation of 4-aminoantipyrine (4-AAP) with modified Trinder's reagent N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3, 5-dimethoxyaniline (DAOS) by H₂O₂.By coupling this mimetic catalytic reaction with the catalytic reaction of glucose oxidase (GOD),glucose can be detected. Under optimum conditions, the calibration graphs for the determination of H₂O₂ and glucose are in the range of 1.0×10^-3-1.0×10^-1 mol/L and 1.0×10^-3-14×10^-3 mol/L respectively. The detection limit is 5.9×10^-4 mol/L for H₂O₂ and is 9.2×10^-4 mol/L for glucose.The feasibility of Mn (Ⅱ) as a HRP mimetic enzyme in practical clinical analysis has been proven in the determination of glucose in human serum. So far, Mn (Ⅱ) is the simplest and the most inexpensive mimetic enzyme.
- Mn (Ⅱ),
- mimetic enzyme,
- spectrophotometry,
- hydrogen peroxide,
- glucose.
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