Citation: Nong Yue HE, Hui Shi GUO, Di YANG, Chun Rong GU, Ji Nan ZHANG. SBA-15 Modified Carbon Paste Electrode for Rapid cTnI Detection with Enhanced Sensitivity[J]. Chinese Chemical Letters, ;2006, 17(2): 235-238. shu

SBA-15 Modified Carbon Paste Electrode for Rapid cTnI Detection with Enhanced Sensitivity

1.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096;
2.
Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029;
3.
Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province, Zhuzhou Institute of Technology, Zhuzhou 412008

Received Date: 7 July 2005

Fund Project: This work was financially supported by NNSFC (60571032, 90406016), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China, and the Trans-Century Training Programme Foundation for the Talents by the Ministry of Education of China.

A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection.A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.
- Cardiac troponin I,
- SBA-15 mesoporous materials,
- carbon paste electrode,
- rapid quantitative immunoassay,
- anodic stripping voltammetric detection,
- nano-gold,
- sensitivity
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