Citation: Mei DU, Sheng JIN, Zhen Quan GUO. A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A (CPA)[J]. Chinese Chemical Letters, ;1999, 10(2): 121-122. shu

A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A (CPA)

a College of Chemistry and Molecular Engineering, Peking University, 100871;
b College of Life Science, Peking University, 100871

Received Date: 7 January 1999

Fund Project: This project was supported by the National Natural Science Foundation of China and the President's Foundation of Peking University.

The monoclonal antibody ID₁₁D₇ to carboxypeptidase A (CPA) was prepared. The inhibitory effect of McAb ID₁₁D₇ on the peptidase activity of CPA was measured. The result reflects that the McAb ID₁₁D₇ can competitively inhibit the peptidase activity of CPA with an apparent inhibitory constant of 6.3 × 10^-9M. Based on this work, the McAb ID₁₁D₇ can be used as idiotypic antigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA.
- Monoclonal antibody,
- carboxypeptidase A (CPA),
- competitive inhibition,
- peptidaseactivity
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