Citation: Shu Xia CAO, Jian Chen ZHANG, Jun XU, Xin Cheng LIAO, Yu Fen ZHAO. Analysis of Hydrolysis Reaction of N-Phosphoryl Phenylalanine by HPLC-ESI-MS/MS[J]. Chinese Chemical Letters, ;2004, 15(7): 817-820. shu

Analysis of Hydrolysis Reaction of N-Phosphoryl Phenylalanine by HPLC-ESI-MS/MS

1.
The Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052;
2.
The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry School of Life Science and Engineering, Tsinghua University, Beijing 100084

Received Date: 6 July 2003

Hydrolysis procedure of N-phosphoryl phenylalanine (DIPP-Phe) was studied by HPLC-ESI-MS/MS. The results showed that (HO)(i-PrO)P(O)Phe was the main intermediate and the hydrolysis of DIPP-Phe also occurred through a penta-coordinate transition state.
- N-Phosphoryl amino acids,
- hydrolysis reaction,
- HPLC-ESI-MS
1. [1]
  Yuan GAO , Yiming LIU , Chunhui WANG , Zhe HAN , Chaoyue FAN , Jie QIU . A hexanuclear cerium oxo cluster stabilized by furoate: Synthesis, structure, and remarkable ability to scavenge hydroxyl radicals. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 491-498. doi: 10.11862/CJIC.20240271
2. [2]
  Jinqi Yang , Xiaoxiang Hu , Yuanyuan Zhang , Lingyu Zhao , Chunlin Yue , Yuan Cao , Yangyang Zhang , Zhenwen Zhao . Direct observation of natural products bound to protein based on UHPLC-ESI-MS combined with molecular dynamics simulation. Chinese Chemical Letters, 2025, 36(5): 110128-. doi: 10.1016/j.cclet.2024.110128
3. [3]
  Huang-Ling Lin , Meng-Zhen Xu , Tian-Ying Gu , Xing-Xing Shi , Mao Le , Zheng Yin , Ming-Hua Zeng . Synthesis, separation and structure of zinc coordination clusters: Proposal of an assembly-disassembly-reassembly process observed from ESI-MS. Chinese Journal of Structural Chemistry, 2025, 44(7): 100602-100602. doi: 10.1016/j.cjsc.2025.100602
4. [4]
  Qiu-Jie Chen , Tian-Ying Gu , Zhao-Bo Hu , Man-Bo Zhang , Zheng Yin , Ming-Hua Zeng . Tandem assembly-disassembly-reassembly of hierarchical Co19 coordination cluster facilitated by in-situ ligand transformation: Crystallography and ESI-MS revealed mechanism. Chinese Journal of Structural Chemistry, 2025, 44(9): 100659-100659. doi: 10.1016/j.cjsc.2025.100659
5. [5]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
6. [6]
  Yanhui Zhong , Peisi Xie , Chengyi Xie , Lei Guo , Weiwei Chen , Shuyi Wang , Xiaoxiao Wang , Fuyue Wang , Zian Lin , Gongke Li , Zongwei Cai . Rigid urea-based structures drive analysis of chiral amino acids. Chinese Chemical Letters, 2026, 37(2): 112039-. doi: 10.1016/j.cclet.2025.112039
7. [7]
  Huidan Geng , Jiuwei Nie , Wei Chen , Chong Zhao , Zikun Yao , Hui Wang , Jiquan Zhang , Lei Tang , Yuanyong Yang . A σ-π bond interchange assisted σ-π cross-metathesis reaction for nonoxidative conversion of methyl ketones to carboxylic acids. Chinese Chemical Letters, 2026, 37(4): 111277-. doi: 10.1016/j.cclet.2025.111277
8. [8]
  Jialin Cai , Yizhe Chen , Ruiwen Zhang , Cheng Yuan , Zeyu Jin , Yongting Chen , Shiming Zhang , Jiujun Zhang . Interfacial Pt-N coordination for promoting oxygen reduction reaction. Chinese Chemical Letters, 2025, 36(2): 110255-. doi: 10.1016/j.cclet.2024.110255
9. [9]
  Yan Guo , Hongtao Bian , Le Yu , Jiani Ma , Yu Fang . Photochemical reaction mechanism of benzophenone protected guanosine at N7 position. Chinese Chemical Letters, 2025, 36(3): 109971-. doi: 10.1016/j.cclet.2024.109971
10. [10]
  Zhen Dai , Linzhi Tan , Yeyu Su , Kerui Zhao , Yushun Tian , Yu Liu , Tao Liu . Site-specific incorporation of reduction-controlled guest amino acids into proteins for cucurbituril recognition. Chinese Chemical Letters, 2024, 35(5): 109121-. doi: 10.1016/j.cclet.2023.109121
11. [11]
  Xiang Huang , Dongzhen Xu , Yang Liu , Xia Huang , Yangfan Wu , Dongmei Fang , Bing Xia , Wei Jiao , Jian Liao , Min Wang . Asymmetric synthesis of difluorinated α-quaternary amino acids (DFAAs) via Cu-catalyzed difluorobenzylation of aldimine esters. Chinese Chemical Letters, 2024, 35(12): 109665-. doi: 10.1016/j.cclet.2024.109665
12. [12]
  Qian Ren , Xue Dai , Ran Cen , Yang Luo , Mingyang Li , Ziyun Zhang , Qinghong Bai , Zhu Tao , Xin Xiao . A cucurbit[8]uril-based supramolecular phosphorescent assembly: Cell imaging and sensing of amino acids in aqueous solution. Chinese Chemical Letters, 2024, 35(12): 110022-. doi: 10.1016/j.cclet.2024.110022
13. [13]
  Min-Hang Zhou , Jun Jiang , Wei-Min He . EDA-complexes-enabled photochemical synthesis of α-amino acids with imines and tetrabutylammonium oxalate. Chinese Chemical Letters, 2025, 36(1): 110446-. doi: 10.1016/j.cclet.2024.110446
14. [14]
  Yi-Xin Zhang , Fang-Qi Zhang , Ao-Pei Peng , Tao Jiang , Ya-Xi Meng , Yang Li , Shuang-Xi Gu , Yuan-Yuan Zhu . Enantioselective recognition of amino acids in water using emission-tunable chiral fluorescent probes. Chinese Chemical Letters, 2026, 37(1): 111500-. doi: 10.1016/j.cclet.2025.111500
15. [15]
  Xiao Tang , Erik V. Van der Eycken , Liangliang Song . Transition metal-catalyzed C-H activation/annulation for the construction of unnatural amino acids and peptides. Chinese Chemical Letters, 2026, 37(2): 111678-. doi: 10.1016/j.cclet.2025.111678
16. [16]
  Bin Dong , Ning Yu , Qiu-Yue Wang , Jing-Ke Ren , Xin-Yu Zhang , Zhi-Jie Zhang , Ruo-Yao Fan , Da-Peng Liu , Yong-Ming Chai . Double active sites promoting hydrogen evolution activity and stability of CoRuOH/Co₂P by rapid hydrolysis. Chinese Chemical Letters, 2024, 35(7): 109221-. doi: 10.1016/j.cclet.2023.109221
17. [17]
  Xian-Fa Jiang , Chongyun Shao , Zhongwen Ouyang , Zhao-Bo Hu , Zhenxing Wang , You Song . Generating electron spin qubit in metal-organic frameworks via spontaneous hydrolysis. Chinese Chemical Letters, 2024, 35(7): 109011-. doi: 10.1016/j.cclet.2023.109011
18. [18]
  Qian Liu , Yi Shi , Kaiya Wang , Xiao-Yu Hu . Tailoring cascade hydrolysis and cyclization efficiency in confined spaces via spatial and electrostatic regulation. Chinese Chemical Letters, 2025, 36(12): 111462-. doi: 10.1016/j.cclet.2025.111462
19. [19]
  Yi Zhou , Yanzhen Liu , Yani Yan , Zonglin Yi , Yongfeng Li , Cheng-Meng Chen . Enhanced oxygen reduction reaction on La-Fe bimetal in porous N-doped carbon dodecahedra with CNTs wrapping. Chinese Chemical Letters, 2025, 36(1): 109569-. doi: 10.1016/j.cclet.2024.109569
20. [20]
  Chenhao Zhang , Qian Zhang , Yezhou Hu , Hanyu Hu , Junhao Yang , Chang Yang , Ye Zhu , Zhengkai Tu , Deli Wang . N-doped carbon confined ternary Pt₂NiCo intermetallics for efficient oxygen reduction reaction. Chinese Chemical Letters, 2025, 36(3): 110429-. doi: 10.1016/j.cclet.2024.110429

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1370)
HTML views(24)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return