Citation: Ru Ding, Chunfei Wang, E Lv, Jingjing Zhao, Xucong Zhou, Yuanyi Li, Ying Wang, Bingxue Li, Lixia Zhang, Jin Zhou. A near-infrared fluorescent probe based on Au³⁺/Pd²⁺ complexes for glutathione sensing: Applications in acute liver injury, hepatocellular carcinoma, and ferroptosis exploration[J]. Chinese Chemical Letters, ;2025, 36(11): 111497. doi: 10.1016/j.cclet.2025.111497 shu

A near-infrared fluorescent probe based on Au³⁺/Pd²⁺ complexes for glutathione sensing: Applications in acute liver injury, hepatocellular carcinoma, and ferroptosis exploration

Ru Ding ^{a, 1} ,
Chunfei Wang ^{b, 1} ,
E Lv ^{a, 1} ,
Jingjing Zhao ^a ,
Xucong Zhou ^a ,
Yuanyi Li ^a ,
Ying Wang ^{c, *,} ,
Bingxue Li ^{a, *,} ,
Lixia Zhang ^a ,
Jin Zhou ^{a, *,}

a.
School of Pharmacy, School of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, China
b.
Weifang People's Hospital, Shandong Second Medical University, Weifang 261041, China
c.
School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao 266071, China

wangying@uor.edu.cn

libingxue@sdsmu.edu.cn

zhoujin@sdsmu.edu.cn

Received Date: 27 April 2025
Revised Date: 19 June 2025
Accepted Date: 23 June 2025
Available Online: 23 June 2025

Figures(4)

Liver diseases, particularly acute alcoholic liver injury (AALI), drug-induced liver injury (DILI), and hepatocellular carcinoma (HCC), have become global public health issues. Glutathione (GSH), as an important antioxidant, plays a crucial role in the liver, and its changes are closely associated with liver injury and the development of liver cancer. Therefore, accurately monitoring GSH variations is critical for understanding liver injury mechanisms, early diagnosis, and treatment evaluation. However, traditional detection methods suffer from insufficient sensitivity and selectivity. To address these challenges, we developed an innovative DR-Au³⁺/DR-Pd²⁺ complex probe that can rapidly and sensitively detect GSH through near-infrared (NIR) fluorescence changes. This probe, with the optimal excitation and emission wavelengths of the probe both located in the NIR region, exhibits excellent selectivity and liver-targeting ability, overcoming the imprecision localization problems of traditional methods. In the AALI and DILI models, the optimized DR-Au³⁺ probe enables real-time monitoring of GSH level fluctuations, providing a powerful tool for early diagnosis of liver injury and dynamic evaluation of therapeutic efficacy. In the DILI and HCC models, the DR-Au³⁺ probe enables visualization and quantitative monitoring of the ferroptosis process, offering new perspectives and approaches for targeted therapy research. The DR-Au³⁺ probe we developed pioneers innovative strategies for establishing accurate diagnostic protocols and individualized therapeutic regimens in hepatic injury and hepatocellular carcinoma management.
- Near-infrared fluorescent probe,
- Glutathione,
- Liver injury,
- Hepatocellular carcinoma,
- Ferroptosis
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

A near-infrared fluorescent probe based on Au3+/Pd2+ complexes for glutathione sensing: Applications in acute liver injury, hepatocellular carcinoma, and ferroptosis exploration

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通讯作者: 陈斌, bchen63@163.com

A near-infrared fluorescent probe based on Au³⁺/Pd²⁺ complexes for glutathione sensing: Applications in acute liver injury, hepatocellular carcinoma, and ferroptosis exploration