Citation: Shujun Liu, Wenfeng Xu, Pengfei Jin, Li-Li Huang. Real-time monitoring of virus infection dynamics in established infection models for mechanism analysis[J]. Chinese Chemical Letters, ;2026, 37(4): 111076. doi: 10.1016/j.cclet.2025.111076 shu

Real-time monitoring of virus infection dynamics in established infection models for mechanism analysis

Shujun Liu ^a ,
Wenfeng Xu ^b ,
Pengfei Jin ^{b, *,} ,
Li-Li Huang ^{a, c, *,}

a.
School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China
b.
Department of Pharmacy, Beijing Hospital, National Center of Gerontology, Beijing Key Laboratory of Assessment of Clinical Drugs Risk and Individual Application (Beijing Hospital), Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China
c.
Tangshan Research Institute, Beijing Institute of Technology, Tangshan 063000, China

j790101@163.com

llhuang@bit.edu.cn

Received Date: 18 January 2025
Revised Date: 7 March 2025
Accepted Date: 12 March 2025
Available Online: 13 March 2025

Figures(13)

Understanding the mechanisms of virus infection is pivotal for the effective prevention and treatment of viral diseases. This review provides a comprehensive overview of the latest advancements in real-time monitoring of viral dynamics within established infection models. We begin by summarizing the recent progress in fluorescent probe and labeling techniques for real-time and in situ virus tracking. Next, we provide an in-depth analysis of the types and characteristics of virus infection models and discuss their respective advantages and limitations in virus tracking. Finally, we detail the recent progress in viral dynamics tracking across different infection models, illustrating how to use these models to monitor virus infection dynamics and discussing the meaningful biological information that can be acquired.
- Virus labeling,
- Fluorescent probe,
- Dynamic tracing,
- Virus infection models,
- Infection mechanisms
1. [1]
  Z.Hong Wu, Y. Jing, H. Wen Xian, et al., Influenza A (H1N1), in: P.X. Lu, B.P. Zhou (Eds.), Diagnostic Imaging of Emerging Infectious Diseases, Springer, Dordrecht, 2016, pp. 57–76.
2. [2]
  E.I. Azhar, D.S.C. Hui, Z.A. Memish, C. Drosten, A. Zumla, Infect. Dis. Clin. N. Am. 33 (2019) 891–905. doi: 10.1016/j.idc.2019.08.001
3. [3]
  S. Hasan, S.A. Ahmad, R. Masood, S. Saeed, J. Fam. Med. Prim. Care 8 (2019) 2189–2201. doi: 10.4103/jfmpc.jfmpc_297_19
4. [4]
  S. Sanche, Y.T. Lin, C.G. Xu, et al., Emerg. Infect. Dis. 26 (2020) 1470–1477. doi: 10.3201/eid2607.200282
5. [5]
  S.R. Stein, S.C. Ramelli, A. Grazioli, et al., Nature 612 (2022) 758–763. doi: 10.1038/s41586-022-05542-y
6. [6]
  M. Rojas, D.M. Monsalve, Y. Pacheco, et al., J. Autoimmun. 106 (2020) 26. doi: 10.2307/j.ctv15d821h.8
7. [7]
  E. de Wit, N. van Doremalen, D. Falzarano, V.J. Munster, Nat. Rev. Microbiol. 14 (2016) 523–534. doi: 10.1038/nrmicro.2016.81
8. [8]
  A.E. Smith, A. Helenius, Science 304 (2004) 237–242. doi: 10.1126/science.1094823
9. [9]
  A. Refaat, M.L. Yap, G. Pietersz, et al., J. Nanobiotechnol. 20 (2022) 450. doi: 10.1186/s12951-022-01648-7
10. [10]
  J.H. Zhang, H.Y. Li, B. Lin, et al., J. Am. Chem. Soc. 143 (2021) 19317–19329. doi: 10.1021/jacs.1c04577
11. [11]
  M. Pirzada, Z. Altintas, Chem. Soc. Rev. 51 (2022) 5805–5841. doi: 10.1039/d1cs01150b
12. [12]
  D. Liu, L. Pan, H.J. Zhai, H.J. Qiu, Y. Sun, Front. Immunol. 14 (2023) 1204730. doi: 10.3389/fimmu.2023.1204730
13. [13]
  S.L. Liu, Z.G. Wang, H.Y. Xie, et al., Chem. Rev. 120 (2020) 1936–1979. doi: 10.1021/acs.chemrev.9b00692
14. [14]
  R. Aviner, J. Frydman, Cold Spring Harb. Perspect. Biol. 12 (2020) a034090. doi: 10.1101/cshperspect.a034090
15. [15]
  W.B. Wang, J.Z. Chen, X.Q. Yu, H.Y. Lan, Int. J. Biol. Sci. 18 (2022) 4704–4713. doi: 10.7150/ijbs.72663
16. [16]
  A. Chaillon, S. Gianella, S. Dellicour, et al., J. Clin. Investig. 130 (2020) 1699–1712. doi: 10.1172/jci134815
17. [17]
  X.L. Ke, C.J. Li, D. Luo, et al., J. Nanobiotechnol. 19 (2021) 295. doi: 10.1109/iciba52610.2021.9688179
18. [18]
  Z.G. Wang, S.L. Liu, D.W. Pang, Acc. Chem. Res. 54 (2021) 2991–3002. doi: 10.1021/acs.accounts.1c00276
19. [19]
  A.X. Ma, C. Yu, M.Y. Zhang, et al., Nano Lett. 24 (2024) 2544–2552. doi: 10.1021/acs.nanolett.3c04600
20. [20]
  A.A. Liu, Z.F. Zhang, E.Z. Sun, et al., ACS Nano 10 (2016) 1147–1155. doi: 10.1021/acsnano.5b06438
21. [21]
  E.A. Rodriguez, R.E. Campbell, J.Y. Lin, et al., Trends Biochem. Sci. 42 (2017) 111–129. doi: 10.1016/j.tibs.2016.09.010
22. [22]
  K.A. Lukyanov, Biochem. Biophys. Res. Commun. 633 (2022) 29–32. doi: 10.1016/j.bbrc.2022.08.089
23. [23]
  J. Kong, Y.F. Wang, W. Qi, et al., Adv. Colloid Interface Sci. 285 (2020) 102286. doi: 10.1016/j.cis.2020.102286
24. [24]
  R.N. Day, M.W. Davidson, Chem. Soc. Rev. 38 (2009) 2887–2921. doi: 10.1039/b901966a
25. [25]
  Y. Klingen, K.K. Conzelmann, S. Finke, J. Virol. 82 (2008) 237–245. doi: 10.1128/JVI.01342-07
26. [26]
  D.M. Shcherbakova, O.M. Subach, V.V. Verkhusha, Angew. Chem. Int. Ed. 51 (2012) 10724–10738. doi: 10.1002/anie.201200408
27. [27]
  M. Sharma, M. Marin, H. Wu, D. Prikryl, G.B. Melikyan, ACS Nano 17 (2023) 17436–17450. doi: 10.1021/acsnano.3c05508
28. [28]
  L. Olivi, C. Bagchus, V. Pool, et al., Nucleic Acids Res. 52 (2024) 5241–5256. doi: 10.1093/nar/gkae283
29. [29]
  K. Nienhaus, G.U. Nienhaus, Chem. Soc. Rev. 43 (2014) 1088–1106. doi: 10.1039/C3CS60171D
30. [30]
  V. Adam, R. Berardozzi, M. Byrdin, D. Bourgeois, Curr. Opin. Chem. Biol. 20 (2014) 92–102. doi: 10.1016/j.cbpa.2014.05.016
31. [31]
  K. Nienhaus, G.U. Nienhaus, RSC Chem. Biol. 2 (2021) 796–814. doi: 10.1039/d1cb00014d
32. [32]
  V.N. Ngo, D.P. Winski, B. Aho, et al., Virology 599 (2024) 110198. doi: 10.1016/j.virol.2024.110198
33. [33]
  F. Pennacchietti, E.O. Serebrovskaya, A.R. Faro, et al., Nat. Methods 15 (2018) 601–604. doi: 10.1038/s41592-018-0052-9
34. [34]
  L.T. Tang, C. Fang, Int. J. Mol. Sci. 23 (2022) 6459. doi: 10.3390/ijms23126459
35. [35]
  X.X. Zhou, H.K. Chung, A.J. Lam, M.Z. Lin, Science 338 (2012) 810–814. doi: 10.1126/science.1226854
36. [36]
  I. Chen, A.Y. Ting, Curr. Opin. Biotechnol. 16 (2005) 35–40. doi: 10.1016/j.copbio.2004.12.003
37. [37]
  D.Y. Orgeirsdottir, J.H. Andersen, M. Perch-Nielsen, et al., Eur. J. Pharm. Sci. 183 (2023) 106400. doi: 10.1016/j.ejps.2023.106400
38. [38]
  Y.K. Gong, L.F. Pan, Tetrahedron Lett. 56 (2015) 2123–2132. doi: 10.1016/j.tetlet.2015.03.065
39. [39]
  N. Vogt, Nat. Methods 18 (2021) 27. doi: 10.1038/s41592-020-01026-x
40. [40]
  M. Minoshima, S.I. Reja, R. Hashimoto, K. Iijima, K. Kikuchi, Chem. Rev. 124 (2024) 6198–6270. doi: 10.1021/acs.chemrev.3c00549
41. [41]
  D. Sivaraman, P. Biswas, L.N. Cella, M.V. Yates, W. Chen, Trends Biotechnol. 29 (2011) 307–313. doi: 10.1016/j.tibtech.2011.02.006
42. [42]
  N.K. Singh, K. Pushpavanam, M. Radhakrishna, ACS Appl. Bio Mater. 7 (2024) 596–608. doi: 10.1021/acsabm.3c00125
43. [43]
  Y.P. Wang, K. Wu, M. Pan, et al., ACS Appl. Mater. Interfaces 12 (2020) 35873–35881. doi: 10.1021/acsami.0c08462
44. [44]
  Y.D. Gang, H.F. Zhou, Y. Jia, et al., Front. Neurosci. 11 (2017) 121.
45. [45]
  W. Wan, Y.A. Huang, Q.X. Xia, et al., Angew. Chem. Int. Ed. 60 (2021) 11335–11343. doi: 10.1002/anie.202015988
46. [46]
  J.A. Prescher, C.R. Bertozzi, Nat. Chem. Biol. 1 (2005) 13–21. doi: 10.1038/nchembio0605-13
47. [47]
  M. Jurkovic, M. Ferger, I. Draskovic, T.B. Marder, I. Piantanida, Pharmaceuticals 16 (2023) 1208. doi: 10.3390/ph16091208
48. [48]
  T. Wang, Z.H. Zheng, X.E. Zhang, H.Z. Wang, Talanta 158 (2016) 179–184. doi: 10.1016/j.talanta.2016.04.052
49. [49]
  C. Bräuchle, T. Endress, A. Zumbusch, et al., ChemPhysChem 3 (2002) 299–303. doi: 10.1002/1439-7641(20020315)3:3<299::AID-CPHC299>3.0.CO;2-R
50. [50]
  G. Seisenberger, M.U. Ried, T. Endress, et al., Science 294 (2001) 1929–1932. doi: 10.1126/science.1064103
51. [51]
  L. Wen, Y. Lin, Z.L. Zhang, et al., Biomaterials 99 (2016) 24–33. doi: 10.1016/j.biomaterials.2016.04.038
52. [52]
  L.L. Huang, K.J. Liu, Q.M. Zhang, et al., Anal. Chem. 89 (2017) 11620–11627. doi: 10.1021/acs.analchem.7b03043
53. [53]
  Y.F. Yang, F.C. Gao, Y.D. Wang, et al., Molecules 27 (2022) 21.
54. [54]
  C. Yu, H.J. Chen, H.Y. Liu, et al., Nano Today 59 (2024) 102527. doi: 10.1016/j.nantod.2024.102527
55. [55]
  D.Y. Jin, P. Xi, B.M. Wang, et al., Nat. Methods 15 (2018) 415–423. doi: 10.1038/s41592-018-0012-4
56. [56]
  H.Y. Liu, Z.G. Wang, S.L. Liu, D.W. Pang, Nat. Protoc. 18 (2023) 458–489. doi: 10.1038/s41596-022-00775-7
57. [57]
  M. Xie, K. Luo, B.H. Huang, et al., Biomaterials 31 (2010) 8362–8370. doi: 10.1016/j.biomaterials.2010.07.063
58. [58]
  S.L. Liu, Z.L. Zhang, Z.Q. Tian, et al., ACS Nano 6 (2012) 141–150. doi: 10.1021/nn2031353
59. [59]
  M. Bally, S. Block, F. Höök, et al., Anal. Bioanal. Chem. 413 (2021) 7157–7178. doi: 10.1007/s00216-021-03510-5
60. [60]
  P. Gao, Z. Xie, M. Zheng, Chin. Chem. Lett. 33 (2022) 1659–1672. doi: 10.1016/j.cclet.2021.09.085
61. [61]
  Y.Q. Li, P.X. Ma, Q. Tao, et al., Sens. Actuator B: Chem. 337 (2021) 129786. doi: 10.1016/j.snb.2021.129786
62. [62]
  Y.X. Xue, C.C. Liu, G. Andrews, J.Y. Wang, Y. Ge, Nano Converg. 9 (2022) 15. doi: 10.1186/s40580-022-00307-9
63. [63]
  G.J. Pang, Y.Y. Zhang, X.Y. Wang, et al., Nano Today 40 (2021) 101264. doi: 10.1016/j.nantod.2021.101264
64. [64]
  Y. Ning, L. Wei, S. Lin, et al., Chin. Chem. Lett. 33 (2022) 4710–4714. doi: 10.1016/j.cclet.2021.12.084
65. [65]
  S. Shen, W. Xu, J. Lu, et al., Chin. Chem. Lett. 35 (2024) 108360. doi: 10.1016/j.cclet.2023.108360
66. [66]
  U.F. Greber, M. Way, Cell 124 (2006) 741–754. doi: 10.1016/j.cell.2006.02.018
67. [67]
  Q. Li, W. Yin, W. Li, et al., Nano Lett. 18 (2018) 7457–7468. doi: 10.1021/acs.nanolett.8b02800
68. [68]
  Y.Z. Liu, X.P. Lü, Z.X. Pan, et al., Chin. Med. J. 126 (2013) 3344–3347. doi: 10.3760/cma.j.issn.0366-6999.20130461
69. [69]
  W. Wang, X. Ai, STAR Protoc. 2 (2021) 100756. doi: 10.1016/j.xpro.2021.100756
70. [70]
  Q.R. Zhang, F.L. Tian, F. Wang, et al., ACS Nano 14 (2020) 7046–7054. doi: 10.1021/acsnano.0c01739
71. [71]
  S.C. Weaver, C. Charlier, N. Vasilakis, M. Lecuit, Annu. Rev. Med. 69 (2018) 395–408. doi: 10.1146/annurev-med-050715-105122
72. [72]
  Y.R. Guo, Q.D. Cao, Z.S. Hong, et al., Mil. Med. Res. 7 (2020) 11.
73. [73]
  D. Primorac, K. Vrdoljak, P. Brlek, et al., Immunology 13 (2022) 848582.
74. [74]
  M.Z.M. Zheng, L.M. Wakim, Mucosal Immunol. 15 (2022) 379–388. doi: 10.1038/s41385-021-00461-z
75. [75]
  S.P. Nobs, A.A. Kolodziejczyk, L. Adler, et al., Nature 532 (2016) 512–516. doi: 10.1038/nature17655
76. [76]
  R.J. Platt, S.D. Chen, Y. Zhou, et al., Cell 159 (2014) 440–455. doi: 10.1016/j.cell.2014.09.014
77. [77]
  N. Liu, E.N. Olson, Circ. Res. 130 (2022) 1827–1850. doi: 10.1161/circresaha.122.320496
78. [78]
  S.A. Chen, S. Sun, D. Moonen, et al., Cell Rep. 27 (2019) 3780–3789. doi: 10.1016/j.celrep.2019.05.103
79. [79]
  X. Li, W. Yin, A. Li, et al., J. Med. Virol. 95 (2023) e28470. doi: 10.1002/jmv.28470
80. [80]
  J.R. Spengler, J. Prescott, H. Feldmann, C.F. Spiropoulou, Curr. Opin. Virol. 25 (2017) 90–96. doi: 10.1016/j.coviro.2017.07.028
81. [81]
  W.H. Yu, J.B. Wang, Y. Yang, et al., J. Med. Virol. 95 (2023) e28846. doi: 10.1002/jmv.28846
82. [82]
  A. Vucetic, A. Lafleur, M. Côté, et al., Front. Cell. Infect. Microbiol. 13 (2023) 1275277. doi: 10.3389/fcimb.2023.1275277
83. [83]
  L. Wang, L. Liu, L. Wang, Rev. Med. Virol. 28 (2018) e1961. doi: 10.1002/rmv.1961
84. [84]
  K.K. Patel, T. Strive, R.N. Hall, et al., Transbound. Emerg. Dis. 69 (2022) e1959–e1971.
85. [85]
  R. Tangwangvivat, S. Chaiyawong, N. Nonthabenjawan, et al., Virol. J. 19 (2022) 162. doi: 10.1186/s12985-022-01888-x
86. [86]
  K.S. Hwang, E.U. Seo, N. Choi, J. Kim, H.N. Kim, Bioact. Mater. 21 (2023) 576–594.
87. [87]
  R. Bhowmick, T. Derakhshan, Y. Liang, et al., Tissue Eng. Part A 24 (2018) 1468–1480. doi: 10.1089/ten.tea.2017.0449
88. [88]
  X.L. Zhu, X.T. Ding, SLAS Discov. 22 (2017) 626–634. doi: 10.1177/2472555217701247
89. [89]
  R. Plebani, H.Q. Bai, L.L. Si, et al., Int. J. Mol. Sci. 23 (2022) 10071. doi: 10.3390/ijms231710071
90. [90]
  Y. Wang, H. Wang, P. Deng, et al., Lab Chip 18 (2018) 3606–3616. doi: 10.1039/c8lc00869h
91. [91]
  P. Wang, L. Jin, M. Zhang, et al., Nat. Biomed. Eng. 8 (2024) 1053–1068.
92. [92]
  S. Satta, S.J. Rockwood, K.D. Wang, et al., Circ. Res. 132 (2023) 1405–1424. doi: 10.1161/circresaha.122.321877
93. [93]
  A. Fedi, C. Vitale, P. Giannoni, G. Caluori, A. Marrella, Sensors 22 (2022) 1517. doi: 10.3390/s22041517
94. [94]
  S. Maji, H. Lee, Int. J. Mol. Sci. 23 (2022) 2662. doi: 10.3390/ijms23052662
95. [95]
  P.Q. Ma, Y. Chen, X.Y. Lai, et al., Macromol. Biosci. 21 (2021) e2100191. doi: 10.1002/mabi.202100191
96. [96]
  H. Liu, Y. Wang, K. Cui, et al., Adv. Mater. 31 (2019) e1902042. doi: 10.1002/adma.201902042
97. [97]
  T. Do, L. Synan, G. Ali, H. Gappa-Fahlenkamp, Stem Cell Res. Ther. 13 (2022) 464. doi: 10.1186/s13287-022-03134-1
98. [98]
  A. Imle, P. Kumberger, N.D. Schnellbächer, et al., Nat. Commun. 10 (2019) 2144. doi: 10.1038/s41467-019-09879-3
99. [99]
  D.A. Ferreira, M. Rothbauer, J.P. Conde, et al., Adv. Sci. 8 (2021) 2003273. doi: 10.1002/advs.202003273
100. [100]
  K. Gold, A.K. Gaharwar, A. Jain, Biomaterials 196 (2019) 2–17. doi: 10.1016/j.biomaterials.2018.07.029
101. [101]
  Y.S. Zhang, A. Arneri, S. Bersini, et al., Biomaterials 110 (2016) 45–59. doi: 10.1016/j.biomaterials.2016.09.003
102. [102]
  S.L. Mi, Z.C. Du, Y.Y. Xu, W. Sun, J. Mater. Chem. B 6 (2018) 6191–6206. doi: 10.1039/c8tb01661e
103. [103]
  J. Yu, S. Lee, J. Song, et al., Nano Converg. 9 (2022) 16. doi: 10.21037/acr-21-70
104. [104]
  S. Lee, J. Lim, J. Yu, et al., Lab Chip 19 (2019) 2071–2080. doi: 10.1039/c9lc00148d
105. [105]
  K. Ronaldson-Bouchard, D. Teles, K. Yeager, et al., Nat. Biomed. Eng. 6 (2022) 351–371. doi: 10.1038/s41551-022-00882-6
106. [106]
  F.F. Wu, D. Wu, Y. Ren, et al., J. Hepatol. 70 (2019) 1145–1158. doi: 10.1016/j.jhep.2018.12.028
107. [107]
  B. Gabbin, V. Meraviglia, M.L. Angenent, et al., Mater. Today Bio 23 (2023) 100818. doi: 10.1016/j.mtbio.2023.100818
108. [108]
  M.J. Rupar, T. Sasserath, E. Smith, et al., Sci. Rep. 13 (2023) 10509. doi: 10.1038/s41598-023-35694-4
109. [109]
  G.D. Vatine, R. Barrile, M.J. Workman, et al., Cell Stem Cell 24 (2019) 995–1005. doi: 10.1016/j.stem.2019.05.011
110. [110]
  M. Zhang, P. Wang, R. Luo, et al., Adv. Sci. 8 (2020) 14. doi: 10.1182/blood-2020-134350
111. [111]
  C. Günther, B. Winner, M.F. Neurath, T.S. Stappenbeck, Gut 71 (2022) 1892–1908. doi: 10.1136/gutjnl-2021-326560
112. [112]
  M. Poletti, K. Arnauts, M. Ferrante, T. Korcsmaros, J. Crohns Colitis 15 (2021) 1222–1235. doi: 10.1093/ecco-jcc/jjaa257
113. [113]
  V. Veninga, E.E. Voest, Cancer Cell 39 (2021) 1190–1201. doi: 10.1016/j.ccell.2021.07.020
114. [114]
  X.Y. Tang, S.S. Wu, D. Wang, et al., Signal Transduct. Target. Ther. 7 (2022) 168. doi: 10.1038/s41392-022-01024-9
115. [115]
  J. Tanaka, H. Senpuku, M. Ogawa, et al., Nat. Cell Biol. 25 (2023) 508. doi: 10.1038/s41556-023-01107-x
116. [116]
  H.X. Xu, D.C. Jiao, A.G. Liu, K.M. Wu, J. Hematol. Oncol. 15 (2022) 58. doi: 10.3390/infrastructures7040058
117. [117]
  K. Salewskij, J.M. Penninger, Circ. Res. 132 (2023) 498–510. doi: 10.1161/circresaha.122.321768
118. [118]
  X.M. Tang, D.X. Xue, T. Zhang, et al., Nat. Cell Biol. 25 (2023) 381–389. doi: 10.1038/s41556-023-01095-y
119. [119]
  C.T. Ekanger, F. Zhou, D. Bohan, et al., Front. Cell. Infect. Microbiol. 12 (2022) 1–23.
120. [120]
  C. Wang, J. Wang, D. Liu, Z.L. Zhang, Chin. Chem. Lett. 35 (2024) 110302. doi: 10.1016/j.cclet.2024.110302
121. [121]
  R. Nishinakamura, Cell Stem Cell 30 (2023) 1017–1027. doi: 10.1016/j.stem.2023.07.011
122. [122]
  H. Wang, X.F. Ning, F. Zhao, H. Zhao, D. Li, Theranostics 14 (2024) 788–818. doi: 10.7150/thno.90492
123. [123]
  Y.P. Zhang, Z.G. Wang, Y.F. Tian, et al., Angew. Chem. Int. Ed. 62 (2023) e202217230. doi: 10.1002/anie.202217230
124. [124]
  H.Y. Liu, Y.S. Hu, C. Yu, et al., Sci. Bull. 69 (2024) 502–511. doi: 10.1016/j.scib.2023.11.020
125. [125]
  S. Murali, R.R. Rustandi, X.W. Zheng, A. Payne, L. Shang, Viruses 14 (2022) 717. doi: 10.3390/v14040717
126. [126]
  C.F. Soon, S.H. Zhang, P.V. Suneetha, et al., Front. Immunol. 10 (2019) 2076. doi: 10.3389/fimmu.2019.02076
127. [127]
  Y.S. Wan, J. Shang, R. Graham, R.S. Baric, F. Li, J. Virol. 94 (2020) e00127-20. doi: 10.1128/JVI.00127-20
128. [128]
  N.L. Meyer, M.S. Chapman, Trends Microbiol. 30 (2022) 432–451. doi: 10.1016/j.tim.2021.09.005
129. [129]
  P.G. Spear, R.J. Eisenberg, G.H. Cohen, Virology 275 (2000) 1–8.
130. [130]
  J. Shang, Y.S. Wan, C.M. Luo, et al., Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 11727–11734. doi: 10.1073/pnas.2003138117
131. [131]
  S. Boersma, H.H. Rabouw, L.J.M. Bruurs, et al., Cell 183 (2020) 1930–1945. doi: 10.1016/j.cell.2020.10.019
132. [132]
  J. Liu, M.Y. Xu, B. Tang, et al., Small 15 (2019) e1803788. doi: 10.1002/smll.201803788
133. [133]
  N. Arhel, A. Genovesio, K.A. Kim, et al., Nat. Methods 3 (2006) 817–824. doi: 10.1038/nmeth928
134. [134]
  X.H. Dong, H. Li, A. Derdowski, et al., Cell 120 (2005) 663–674. doi: 10.1016/j.cell.2004.12.023
135. [135]
  M.J. Lehmann, N.M. Sherer, C.B. Marks, M. Pypaert, W. Mothes, J. Cell Biol. 170 (2005) 317–325. doi: 10.1083/jcb.200503059
136. [136]
  D. Chen, Q.X. Zheng, L. Sun, et al., Dev. Cell 56 (2021) 3250–3263. doi: 10.1016/j.devcel.2021.10.006
137. [137]
  Y.T. Liu, S. Shivakoti, F. Jia, et al., Cell Discov. 6 (2020) 2.
138. [138]
  G. Wang, D. Zhang, R. Orchard, D.C. Hancks, T.A. Reese, Nature 616 (2023) 152–158. doi: 10.1038/s41586-023-05851-w
139. [139]
  J. Moshiri, A.R. Craven, S.B. Mixon, M.R. Amieva, K. Kirkegaard, Nat. Microbiol. 8 (2023) 629–639. doi: 10.1038/s41564-023-01339-5
140. [140]
  D. Choi, E. Park, K.E. Kim, et al., Cancer Res. 80 (2020) 3130–3144. doi: 10.1158/0008-5472.can-19-3105
141. [141]
  E. Morita, W.I. Sundquist, Annu. Rev. Cell Dev. Biol. 20 (2004) 395–425. doi: 10.1146/annurev.cellbio.20.010403.102350
142. [142]
  A.L. Pinto, R.K. Rai, J.C. Brown, et al., Nat. Commun. 13 (2022) 1609. doi: 10.1038/s41467-022-29255-y
143. [143]
  L.J. Wu, D.N. Jin, D. Wang, et al., Protein Cell 13 (2022) 120–140. doi: 10.1007/s13238-020-00764-0
144. [144]
  S.W. Yoo, A.A. Waheed, P. Deme, et al., Proc. Natl. Acad. Sci. U. S. A. 120 (2023) e2219543120. doi: 10.1073/pnas.2219543120
145. [145]
  M. Perlman, M.D. Resh, Traffic 7 (2006) 731–745. doi: 10.1111/j.1398-9219.2006.00428.x
146. [146]
  X. Sewald, N. Motamedi, W. Mothes, Curr. Opin. Cell Biol. 41 (2016) 81–90. doi: 10.1016/j.ceb.2016.04.008
147. [147]
  S.A.J. Guagliardo, T. Smith, D.H. Hamer, et al., Emerg. Infect. Dis 30 (2024) 2381–2384.
148. [148]
  T. Wang, P.H. Li, Y. Zhang, et al., Theranostics 10 (2020) 6430–6447. doi: 10.7150/thno.43177
149. [149]
  I. Ullah, J. Prevost, M.S. Ladinsky, et al., Immunity 54 (2021) 2143–2158. doi: 10.1016/j.immuni.2021.08.015
150. [150]
  J.A. Pulit-Penaloza, N. Brock, J.A. Belser, et al., Emerg. Microbes Infect. 13 (2024) 2332667. doi: 10.1080/22221751.2024.2332667
151. [151]
  J.H. Kwon, K. Bertran, D.H. Lee, et al., Emerg. Microbes Infect. 12 (2023) 2218945. doi: 10.1080/22221751.2023.2218945
152. [152]
  A.X. Han, S.P.J. de Jong, C.A. Russell, Nat. Rev. Microbiol. 21 (2023) 805–817. doi: 10.1038/s41579-023-00945-8
153. [153]
  H. Pan, W.J. Li, X.J. Yao, et al., Small 13 (2017) 1604036. doi: 10.1002/smll.201604036
154. [154]
  U.M. Ashraf, A.A. Abokor, J.M. Edwards, et al., Physiol. Genom. 53 (2021) 51–60. doi: 10.1152/physiolgenomics.00087.2020
155. [155]
  E. Mitsi, M.O. Diniz, J. Reiné, et al., Nat. Commun. 14 (2023) 6815. doi: 10.1038/s41467-023-42433-w
156. [156]
  C.G. Wang, S.J. Liu, C.Y. Li, et al., ACS Appl. Mater. Interfaces 16 (2024) 60045–60055. doi: 10.1021/acsami.4c15125
157. [157]
  A.A. Salahudeen, S.S. Choi, A. Rustagi, et al., Nature 588 (2020) 670–675. doi: 10.1038/s41586-020-3014-1
158. [158]
  S. Herfst, M. Imai, Y. Kawaoka, R.A.M. Fouchier, Curr. Top. Microbiol. Immunol. 385 (2014) 137–155. doi: 10.1007/82_2014_387
159. [159]
  C.C. Wang, K.A. Prather, J. Sznitman, et al., Science 373 (2021) eabd9149. doi: 10.1126/science.abd9149
160. [160]
  Q. Chen, Y.Y. Liu, J.P. Ren, et al., eLife 10 (2021) e64603. doi: 10.7554/eLife.64603
161. [161]
  X. Jiang, X. Meng, Q. Hu, et al., Chin. J. Zoonoses 34 (2018) 558–562. doi: 10.3390/en11030558
162. [162]
  Y.Z. Liu, S. Maya, A. Ploss, Viruses 13 (2021) 777. doi: 10.3390/v13050777
163. [163]
  K. Shifflett, A. Marzi, Virol. J. 16 (2019) 165. doi: 10.1186/s12985-019-1272-z
164. [164]
  G. Forlani, M. Shallak, R.S. Accolla, M.G. Romanelli, Int. J. Mol. Sci. 22 (2021) 8001. doi: 10.3390/ijms22158001
165. [165]
  J. Geiser, G. Boivin, S. Huang, et al., Viruses 13 (2021) 139. doi: 10.3390/v13010139
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