Citation: Ze Hu, Yaqun Xu, Hao Wang, Gao-Chao Fan, Xiliang Luo. Self-powered anti-interference photoelectrochemical immunosensor based on Au/ZIS/CIS heterojunction photocathode with zwitterionic peptide anchoring[J]. Chinese Chemical Letters, ;2022, 33(11): 4750-4755. doi: 10.1016/j.cclet.2021.12.088 shu

Self-powered anti-interference photoelectrochemical immunosensor based on Au/ZIS/CIS heterojunction photocathode with zwitterionic peptide anchoring

Ze Hu ^a ,
Yaqun Xu ^a ,
Hao Wang ^a ,
Gao-Chao Fan ^{a, b, *,} ,
Xiliang Luo ^{a, *,}

a.
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
b.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

gcfan@qust.edu.cn

xiliangluo@qust.edu.cn

Received Date: 23 October 2021
Revised Date: 22 November 2021
Accepted Date: 31 December 2021
Available Online: 7 January 2022

Figures(5)

Accurate detection of important biomarkers with ultra-low levels in complex biological matrix is one of the frontier scientific issues because of possible signal interference of potential reductive agents and protein molecules. Herein, a self-powered anti-interference photoelectrochemical (PEC) immunosensor was explored for sensitive and specific detection of model target of cardiac troponin I (cTnI). Specifically, a novel ternary heterojunction served as the photocathode to offer a remarkable current output and a zwitterionic peptide was introduced to build a robust antifouling biointerface. CuInS₂ (CIS) film with porous network nanostructure was first prepared and then modified in order with ZnIn₂S₄ (ZIS) nanocrystals and Au nanoparticles to fabricate the Au/ZIS/CIS heterojunction photocathode. After capture cTnI antibody (Ab) was immobilized, the zwitterionic peptide KAEAKAEAPPPPC was then anchored to compete the immunosensor. The elaborated PEC immunosensor exhibited high sensitivity for target cTnI antigen (Ag) detection, with good anti-interference against reductive agents and nonspecific proteins. This integration strategy of heterojunction photocathode with zwitterionic peptide provides a new sight to develop advanced PEC immunosensors applying in practical biosamples.
- Photoelectrochemistry,
- Immunoassay,
- Zwitterionic peptide,
- Photocathode,
- Anti-interference
1. [1]
  J.A. Ludwig, J.N. Weinstein, Nat. Rev. Cancer 5 (2005) 845–856. doi: 10.1038/nrc1739
2. [2]
  C.L. Sawyers, Nature 452 (2008) 548–552. doi: 10.1038/nature06913
3. [3]
  L. Wu, X.G. Qu, Chem. Soc. Rev. 44 (2015) 2963–2997. doi: 10.1039/C4CS00370E
4. [4]
  H. Deng, Y. Chai, R. Yuan, Y. Yuan, Anal. Chem. 92 (2020) 8364–8370. doi: 10.1021/acs.analchem.0c00918
5. [5]
  Y. Fu, K. Zou, M. Liu, et al., Anal. Chem. 92 (2020) 1189–1196. doi: 10.1021/acs.analchem.9b04319
6. [6]
  Q. Zhang, Y. Fu, K. Xiao, et al., Anal. Chem. 93 (2021) 6801–6807. doi: 10.1021/acs.analchem.1c00746
7. [7]
  F. Mo, M. Han, X. Weng, et al., Anal. Chem. 93 (2021) 1764–1770. doi: 10.1021/acs.analchem.0c04521
8. [8]
  Y. Qin, J. Wen, L. Zheng, et al., Nano Lett. 21 (2021) 1879–1887. doi: 10.1021/acs.nanolett.1c00076
9. [9]
  M. Chen, C. Wang, H. Meng, F. Mo, Y.Z. Fu, Chem. Commun. 56 (2020) 2300–2303. doi: 10.1039/C9CC09721J
10. [10]
  W.S. Li, G.C. Fan, F.X. Gao, et al., Biosens. Bioelectron. 127 (2019) 64–71. doi: 10.1016/j.bios.2018.11.043
11. [11]
  J.T. Cao, B. Wang, Y.X. Dong, et al., ACS Sens. 3 (2018) 1087–1092. doi: 10.1021/acssensors.8b00332
12. [12]
  Y.X. Lin, Q. Zhou, D.P. Tang, R. Niessner, D. Knopp, Anal. Chem. 89 (2017) 5637–5645. doi: 10.1021/acs.analchem.7b00942
13. [13]
  H.M. Yang, G.Q. Sun, L.N. Zhang, et al., Sens. Actuators B: Chem. 234 (2016) 658–666. doi: 10.1016/j.snb.2016.05.039
14. [14]
  T. Hisatomi, J. Kubota, K. Domen, Chem. Soc. Rev. 43 (2014) 7520–7535. doi: 10.1039/C3CS60378D
15. [15]
  G.C. Fan, X.M. Shi, J.R. Zhang, J.J. Zhu, Anal. Chem. 88 (2016) 10352–10356. doi: 10.1021/acs.analchem.6b03473
16. [16]
  Z.L. Li, H.M. Yang, M.S. Hu, et al., ACS Appl. Mater. Interfaces 12 (2020) 17177–17184. doi: 10.1021/acsami.9b22558
17. [17]
  Y.H. Zhu, K. Yan, Z.W. Xu, J.N. Wu, J.D. Zhang, Biosens. Bioelectron. 131 (2019) 79–87. doi: 10.1016/j.bios.2019.02.008
18. [18]
  W.X. Dai, L. Zhang, W.W. Zhao, et al., Anal. Chem. 89 (2017) 8070–8078. doi: 10.1021/acs.analchem.7b01557
19. [19]
  G.C. Fan, X.M. Shi, J.R. Zhang, J.J. Zhu, Anal. Chem. 88 (2016) 10352–10356. doi: 10.1021/acs.analchem.6b03473
20. [20]
  C. Jiang, G. Wang, R. Hein, et al., Chem. Rev. 120 (2020) 3852–3889. doi: 10.1021/acs.chemrev.9b00739
21. [21]
  C. Blaszykowski, S. Sheikh, M. Thompson, Chem. Soc. Rev. 41 (2012) 5599–5612. doi: 10.1039/c2cs35170f
22. [22]
  B. Liu, X. Liu, S. Shi, et al., Acta Biomater. 40 (2016) 100–118. doi: 10.1016/j.actbio.2016.02.035
23. [23]
  B.E.I. Ramakers, J.C.M. van Hest, D.W.P.M. Löwik, Chem. Soc. Rev. 43 (2014) 2743–2756. doi: 10.1039/c3cs60362h
24. [24]
  H.J. Ye, L.B. Wang, R.L. Huang, et al., ACS Appl. Mater. Interfaces 7 (2015) 22448–22457. doi: 10.1021/acsami.5b06500
25. [25]
  G. Wang, X. Su, Q. Xu, et al., Biosens. Bioelectron. 101 (2018) 129–134. doi: 10.1016/j.bios.2017.10.024
26. [26]
  C.D. Beyer, M.L. Reback, N. Heinen, et al., Langmuir 36 (2020) 10996–11004. doi: 10.1021/acs.langmuir.0c01790
27. [27]
  X. Han, S. Li, Z. Peng, A.M. Othman, R. Leblanc, ACS Sens. 1 (2016) 106–114. doi: 10.1021/acssensors.5b00318
28. [28]
  D. Fan, C. Bao, M.S. Khan, et al., Biosens. Bioelectron. 106 (2018) 14–20. doi: 10.1016/j.bios.2018.01.050
29. [29]
  K. Guo, Z. Liu, J. Han, et al., Phys. Chem. Chem. Phys. 16 (2014) 16204–16213. doi: 10.1039/C4CP01971G
30. [30]
  Y.M. Tang, J.H. Yun, L.Z. Wang, R. Amal, Y.H. Ng, Dalton Trans. 44 (2015) 7127–7130. doi: 10.1039/C5DT00429B
31. [31]
  L. Zhou, X. Yang, B. Yang, et al., J. Power Sources 272 (2014) 639–646. doi: 10.1016/j.jpowsour.2014.06.172
32. [32]
  J. Han, Z. Liu, K. Guo, et al., Appl. Catal. B 163 (2015) 179–188. doi: 10.1016/j.apcatb.2014.07.040
33. [33]
  Q. Liu, H. Lu, Z.W. Shi, et al., ACS Appl. Mater. Interfaces 6 (2014) 17200–17207. doi: 10.1021/am505015j
34. [34]
  X. Guo, Y. Peng, G. Liu, et al., J. Phys. Chem. C 124 (2020) 5934–5943. doi: 10.1021/acs.jpcc.9b11623
35. [35]
  B.K. Patra, S. Khilari, D. Pradhan, N. Pradhan, Chem. Mater. 28 (2016) 4358–4366. doi: 10.1021/acs.chemmater.6b01357
36. [36]
  S.K. Dutta, S.K. Mehetor, N. Pradhan, J. Phys. Chem. Lett. 6 (2015) 936–944. doi: 10.1021/acs.jpclett.5b00113
37. [37]
  X. Yu, A. Shavel, X. An, et al., J. Am. Chem. Soc. 136 (2014) 9236–9239. doi: 10.1021/ja502076b
38. [38]
  A.K. Nowinski, F. Sun, A.D. White, A.J. Keefe, S. Jiang, J. Am. Chem. Soc. 134 (2012) 6000–6005. doi: 10.1021/ja3006868
39. [39]
  N. Liu, N. Hui, J.J. Davis, X. Luo, ACS Sens. 3 (2018) 1210–1216. doi: 10.1021/acssensors.8b00318
40. [40]
  L. Yuan, L. Liu, Sens. Actuators B: Chem. 344 (2021) 130232. doi: 10.1016/j.snb.2021.130232
41. [41]
  P.H. Chang, C.C. Weng, B.R. Li, Y.K. Li, Biosens. Bioelectron. 151 (2020) 111969. doi: 10.1016/j.bios.2019.111969
42. [42]
  S. Palanisamy, D.S. Raja, B. Subramani, T.H. Wu, Y.M. Wang, ACS Appl. Mater. Interfaces 12 (2020) 32468–32476. doi: 10.1021/acsami.0c09086
43. [43]
  W.S. Chang, P. Li, S. Kakade, et al., Nanoscale 12 (2020) 12568–12577. doi: 10.1039/D0NR02234A
44. [44]
  H. Chi, Q. Han, T. Chi, et al., Biosens. Bioelectron. 132 (2019) 1–7. doi: 10.1016/j.bios.2019.02.048
45. [45]
  X. Tan, L. Zhang, Q. Tang, G. Zheng, H. Li, Microchim. Acta 186 (2019) 280. doi: 10.1007/s00604-019-3375-z
46. [46]
  D. Lou, L. Fan, Y. Cui, et al., Anal. Chem. 90 (2018) 6502–6508. doi: 10.1021/acs.analchem.7b05410
47. [47]
  R. Akter, B. Jeong, Y.M. Lee, J.S. Choi, M.A. Rahman, Biosens. Bioelectron. 91 (2017) 637–643. doi: 10.1016/j.bios.2017.01.021
48. [48]
  M. Negahdary, M. Behjati-Ardakani, N. Sattarahmady, H. Yadegari, H. Heli, Sens. Actuators B: Chem. 252 (2017) 62–71. doi: 10.1016/j.snb.2017.05.149
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