Citation: Yang Yangyang, Zhang Chen, Pan Rizhao, Zhang Shiwei, Yao Shengtao, Tang Yao, Zhu Weilong, Wang Liyue, Zhu Weiping, Xu Yufang, Qian Xuhong. A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity[J]. Chinese Chemical Letters, ;2020, 31(1): 125-128. doi: 10.1016/j.cclet.2019.04.037 shu

A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

Yang Yangyang ^a,*, ,
Zhang Chen ^a ,
Pan Rizhao ^a ,
Zhang Shiwei ^a ,
Yao Shengtao ^a ,
Tang Yao ^a ,
Zhu Weilong ^a ,
Wang Liyue ^a ,
Zhu Weiping ^a ,
Xu Yufang ^a,*, ,
Qian Xuhong ^a,b,**,

a.
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
b.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

triyang@ecust.edu.cn

yfxu@ecust.edu.cn

xhqian@ecust.edu.cn

Received Date: 12 March 2019
Revised Date: 15 April 2019
Accepted Date: 15 April 2019
Available Online: 16 January 2019

Figures(5)

Alkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.
- Fluorescent probe,
- Alkaline phosphatase,
- Ratiometric,
- ESIPT,
- Biomarker
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