Citation: Wang Yueqi, Li Changjian, Du Libo, Liu Yang. A reactive oxygen species-responsive dendrimer with low cytotoxicity for efficient and targeted gene delivery[J]. Chinese Chemical Letters, ;2020, 31(1): 275-280. doi: 10.1016/j.cclet.2019.03.040 shu

A reactive oxygen species-responsive dendrimer with low cytotoxicity for efficient and targeted gene delivery

Wang Yueqi ^a,b,1 ,
Li Changjian ^a,b,1 ,
Du Libo ^a,*, ,
Liu Yang ^a,*,

a.
State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
b.
Graduate School, University of Chinese Academy of Sciences, Beijing 100049, China

dulibo@iccas.ac.cn

yliu@iccas.ac.cn

Received Date: 14 February 2019
Revised Date: 17 March 2019
Accepted Date: 25 March 2019
Available Online: 25 January 2019

Figures(5)

Nonviral vectors have been attracting more attention for several advantages in gene delivery and the development of nonviral gene carriers with high delivery efficiency and low cytotoxicity has long been a key project. Starburst polyamidoamine dendrimers are a class of synthetic polymers with unique structural and physical characteristics. However, when they are used as gene carrier, the gene transfection efficiency is not satisfactory. Herein, a novel thioketal-core polyamidoamine dendrimer (i.e., ROS-PAMAM) was synthesized and characterized. Compared to ethylenediamine-core dendrimers or widely used cationic polymers of polyetherimide, ROS-PAMAM showed lower cytotoxicity. Moreover, ROS-PAMAM demonstrated reactive oxygen species responsive characteristics, which can facilitate the release of siRNA in the tumor microenvironment. In vitro gene transfection experiments based on A549 cells confirmed that siRNA/ROS-PAMAM exhibits high gene transfection efficiency. It is concluded that ROS-PAMAM shows great potential as a generalizable vehicle for gene therapy applications.
- Dendrimer,
- Reactive oxygen species,
- Cancer,
- Gene therapy,
- Low cytotoxicity
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沈阳化工大学材料科学与工程学院沈阳 110142