Citation: Ke Qin Deng Chun Xiang Li, Yu Lin Ling, Guo Rong Xu, Xiao Fang Li. Fabrication of poly (2,6-pyridinedicarboxylic acid)/MWNTs modified electrode for simultaneous determination of guanine and adenine in DNA[J]. Chinese Chemical Letters, ;2011, 22(8): 981-984. doi: 10.1016/j.cclet.2011.01.024 shu

Fabrication of poly (2,6-pyridinedicarboxylic acid)/MWNTs modified electrode for simultaneous determination of guanine and adenine in DNA

Chemistry and Chemical Engineering College, Hunan University of Science and Technology, Xiangtan 411201, China

Received Date: 15 November 2010

Fund Project: This project was supported by National Natural Science Foundation of China (No. 2097104), the Key Project of Chinese Ministry of Education (No. 210146) and the Foundation of Hunan Provincial Department of Education (No. 08c341).

The fabrication of poly (2,6-pyridinedicarboxylic acid)/MWNTs modified glass electrode (PPDA/MWNTs/GCE) was proposed and used for individual or simultaneous determination of guanine and adenine. The performances of the PPDA/MWNTs/GCE were characterized with cyclic voltammetry (CV). The modified electrode exhibited enhanced electrocatalytic behavior and good stability for the detection of guanine and adenine. Differential pulse voltammetry (DPV) was used to determine the concentration of guanine, adenine. The detection limit (S/N=3) for guanine and adenine was 0.045 μmol/L and 0.05 μmol/L, respectively. The electrochemical method for the measurement of guanine and adenine in calf thymus DNA was also developed with this modified electrode and the result was satisfactory.
- Multi-wall carbon nanotubes,
- 2,6-Pyridinedicarboxylic acid,
- Guanine,
- Adenine,
- Calf thymus dsDNA
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