Citation: Dun Wan Zhu, Jin Gen Bo, Hai Ling Zhang, Wen Guang Liu, Xi Gang Leng, Cun Xian Song, Yu Ji Yin, Li Ping Song, Lan Xia Liu, Lin Mei, Xiu Lan Li, Yang Zhang, Kang De Yao. Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier[J]. Chinese Chemical Letters, ;2007, 18(11): 1407-1410. doi: 10.1016/j.cclet.2007.09.006 shu

Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier

a Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China;
b Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;
c Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China

Received Date: 16 April 2007

Fund Project: The authors are indebted to the financial supports from National Natural Science Foundation of China (No.50233020,30300086) and the financial support from Natural Science Foundation of Tianjin (No.05YFJMJC10200).

N-Methylene phosphonic chitosan (NMPCS), an amphiphilic macromolecule with powerful chelating ability of Ca²⁺ ions, was synthesized and characterized. The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR, ¹³C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy (AFM) and complexes with mean size of 80-210 nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan.
- Non-viral vector,
- Gene delivery,
- N-Methylene phosphonic chitosan
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