Citation: Dong Li, Yang-ping Wen, Jing-kun Xu, Hao-hua He, Ming Liu. AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX[J]. Chinese Journal of Polymer Science, ;2012, 30(5): 705-718. doi: 10.1007/s10118-012-1167-6 shu

AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX

Received Date: 30 November 2011
Revised Date: 12 March 2012

Fund Project: This work was financially supported by the NSFC (Nos. 50963002, 51073074), Key Projects in the National Science Technology Pillar Program in the Eleventh Five-year Plan Period (Nos. 2006BAD02A04, 2006BAD01A01), Jiangxi Provincial Department of Science and Technology (2006BAD01A01-2-5).

The biocompatiable and low-toxic poly(thiophene-3-acetic acid) (PTAA) matrix was successfully electrosynthesized in ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF6) in comparison with the electrosynthesis of PTAA matrix in acetonitrile (ACN). Ascorbate oxidase (AO) was used as a model for the development and application of biosensor. Vitamin C (VC) biosensors were facilely fabricated by the covalent immobilization of AO molecules on PTAA matrices electrosynthesized in ACN containing tetrabutylammonium tetrafluoroborate and BmimPF6, respectively. Electrochemical impedance spectroscopy, scanning electron microscopy and FTIR spectroscopy indicated that AO molecules were covalently immobilized on PTAA matrices. Parameters of the as-obtained biosensors such as working potential, pH and temperature have been optimized. The amperometric biosensor based on PTAA matrix electrosynthesized in BmimPF6 exhibited wider linear range, lower detection limit, higher sensitivity and bioaffinity, and better operational and storage stability than that electrosynthesized in ACN under optimal conditions. The as-obtained biosensor based on PTAA matrix electrosynthesized in BmimPF6 was employed for the detection of VC content in commercial juices, and the result was close to the data given by manufacturers. Excellent results indicate that the PTAA matrix electrosynthesized in ionic liquid is a promising platform for the covalent immobilization of biologically-active species and the development of biosensors.
- Poly(thiophene-3-acetic acid)
1. [1]
1. [1]
  Lifang HE , Yaoze LUO , Qiying SHI , Jianxin TANG , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and properties of hydrated tricyclohexyltin theophylline-7-acetic acid complex. Chinese Journal of Inorganic Chemistry, 2026, 42(3): 632-640. doi: 10.11862/CJIC.20250244
2. [2]
  Weijian Zhang , Xianyu Deng , Liying Wang , Jian Wang , Xiuting Guo , Lianggui Huang , Xinyi Wang , Jun Wu , Linjia Jiang . Poly(ferulic acid) nanocarrier enhances chemotherapy sensitivity of acute myeloid leukemia by selectively targeting inflammatory macrophages. Chinese Chemical Letters, 2024, 35(9): 109422-. doi: 10.1016/j.cclet.2023.109422
3. [3]
  Qiang Luo , Jinfeng Sun , Zhibo Li , Bin Liu , Jianxun Ding . Thermo-sensitive poly(amino acid) hydrogel mediates cytoprotection through an antioxidant mechanism. Chinese Chemical Letters, 2025, 36(7): 110433-. doi: 10.1016/j.cclet.2024.110433
4. [4]
  Qi Wei , Hua Xin , Xiaolong Wang , Changjuan Qin , Yuanzhen Su , Di Li , Jianxun Ding . Unimolecular chiral poly(amino acid)s as adjuvants of nanovaccines for augmented cancer immunotherapy. Chinese Chemical Letters, 2025, 36(11): 111477-. doi: 10.1016/j.cclet.2025.111477
5. [5]
  Weixia Wang , Yongji Zhang , Fei Zhao , Libo Wang , Fanpeng Meng , Shuchao Qin , Jinsheng Zhao . Engineering π-delocalized network in fused-thiophene based polymers/g-C3N4 S-scheme heterojunctions for photocatalytic H2 evolution. Chinese Journal of Structural Chemistry, 2026, 45(3): 100833-100833. doi: 10.1016/j.cjsc.2025.100833
6. [6]
  Haojie Song , Laiyu Luo , Siyu Wang , Guo Zhang , Baojiang Jiang . Advances in poly(heptazine imide)/poly(triazine imide) photocatalyst. Chinese Chemical Letters, 2024, 35(10): 109347-. doi: 10.1016/j.cclet.2023.109347
7. [7]
  Zhen Liu , Zhi-Yuan Ren , Chen Yang , Xiangyi Shao , Li Chen , Xin Li . Asymmetric alkenylation reaction of benzoxazinones with diarylethylenes catalyzed by B(C₆F₅)₃/chiral phosphoric acid. Chinese Chemical Letters, 2024, 35(5): 108939-. doi: 10.1016/j.cclet.2023.108939
8. [8]
  Feng Wu , Yuanjiu Xiao , Mengran Wei , Guoqiang Wang , Jian-Jun Feng . Lewis acid catalyzed enantioselective dearomative (3+2) cycloaddition of aromatic N-heterocycles with bicyclobutanes. Chinese Chemical Letters, 2026, 37(3): 111963-. doi: 10.1016/j.cclet.2025.111963
9. [9]
  Jing FENG , Renshu WANG , Hu WANG , Hailong LIU . Co(Ⅱ) and Ni(Ⅱ) complexes of 3,3-diphenylpropionic acid and 2,2′-dipyridylamine: Structures and biological activities. Chinese Journal of Inorganic Chemistry, 2026, 42(3): 617-631. doi: 10.11862/CJIC.20250265
10. [10]
  Weizhong LING , Jingyi LIN , Jianglin ZHU , Yuyi LIANG , Shanshan DAI , Yu LI . Syntheses, structures, and catalytic performances of complexes with 4,4′-dihydroxy-[1,1′-biphenyl]-3,3′-dicarboxylic acid ligands. Chinese Journal of Inorganic Chemistry, 2026, 42(1): 152-160. doi: 10.11862/CJIC.20250204
11. [11]
  Yue Li , Qianyu Ding , Wansheng Liu , Yimeng Sun , Liyao Liu , Ye Zou , Yutao Cui , Jia Zhu , Chongan Di , Daoben Zhu . Bipyridine-bridged Φ-shaped cyclo[8]thiophene[2]pyrrole: Synthesis and fluorescence properties. Chinese Chemical Letters, 2026, 37(2): 111989-. doi: 10.1016/j.cclet.2025.111989
12. [12]
  Xun Zhu , Chenchen Zhang , Yingying Li , Yin Lu , Na Huang , Dawei Wang . Degradation of perfluorooctanoic acid by inductively heated Fenton-like process over the Fe₃O₄/MIL-101 composite. Chinese Chemical Letters, 2024, 35(12): 109753-. doi: 10.1016/j.cclet.2024.109753
13. [13]
  Junying LI , Xinyan CHEN , Xihui DIAO , Muhammad Yaseen , Chao CHEN , Hao WANG , Chuansong QI , Wei LI . Chiral fluorescent sensor Tb³⁺@Cd-CP based on camphoric acid for the enantioselective recognition of R- and S-propylene glycol. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2497-2504. doi: 10.11862/CJIC.20240084
14. [14]
  Yuexiang Liu , Xiangqiao Yang , Tong Lin , Guantian Yang , Xiaoyong Xu , Bubing Zeng , Zhong Li , Weiping Zhu , Xuhong Qian . Efficient continuous synthesis of 2-[3-(trifluoromethyl)phenyl]malonic acid, a key intermediate of Triflumezopyrim, coupling with esterification-condensation-hydrolysis. Chinese Chemical Letters, 2025, 36(1): 109747-. doi: 10.1016/j.cclet.2024.109747
15. [15]
  Ao Sun , Zipeng Li , Shuchun Li , Xiangbao Meng , Zhongtang Li , Zhongjun Li . Stereoselective synthesis of α-3-deoxy-D-manno-oct-2-ulosonic acid (α-Kdo) derivatives using a C3-p-tolylthio-substituted Kdo fluoride donor. Chinese Chemical Letters, 2025, 36(3): 109972-. doi: 10.1016/j.cclet.2024.109972
16. [16]
  Runze Liu , Yankai Bian , Weili Dai . Qualitative and quantitative analysis of Brønsted and Lewis acid sites in zeolites: A combined probe-assisted 1H MAS NMR and NH3-TPD investigation. Chinese Journal of Structural Chemistry, 2024, 43(4): 100250-100250. doi: 10.1016/j.cjsc.2024.100250
17. [17]
  Xinxin Liang , Yongqian Cui , Qingyun Tian , Tingting Jia , Qibing Dong , Ximing Li , Ting Gao , Chao Ma , Chuanyi Wang . Photoreforming polylactic acid plastics into pyruvate over CdS/Bi₄Ti₃O₁₂ S-scheme heterojunction: Successive removal of hydrogen from α-C. Chinese Chemical Letters, 2026, 37(2): 111097-. doi: 10.1016/j.cclet.2025.111097
18. [18]
  Xinyu Liu , Jialin Yang , Zonglin He , Jiaoyan Ai , Lina Song , Baohua Liu . Linear polyurethanes with excellent comprehensive properties from poly(ethylene carbonate) diol. Chinese Chemical Letters, 2025, 36(1): 110236-. doi: 10.1016/j.cclet.2024.110236
19. [19]
  Chong Liu , Ling Li , Jiahui Gao , Yanwei Li , Nazhen Zhang , Jing Zang , Cong Liu , Zhaopei Guo , Yanhui Li , Huayu Tian . The study of antibacterial activity of cationic poly(β-amino ester) regulating by amphiphilic balance. Chinese Chemical Letters, 2025, 36(2): 110118-. doi: 10.1016/j.cclet.2024.110118
20. [20]
  Chen Lian , Si-Han Zhao , Hai-Lou Li , Xinhua Cao . A giant Ce-containing poly(tungstobismuthate): Synthesis, structure and catalytic performance for the decontamination of a sulfur mustard simulant. Chinese Chemical Letters, 2024, 35(10): 109343-. doi: 10.1016/j.cclet.2023.109343

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1620)
HTML views(30)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return