引用本文:
Jun YAO, Yi LIU, Tian Zhi WANG, Jian Ben LIU, Peng LIU, Zi Niu YU. Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase[J]. Chinese Chemical Letters,
2003, 14(6): 619-622.
Citation: Jun YAO, Yi LIU, Tian Zhi WANG, Jian Ben LIU, Peng LIU, Zi Niu YU. Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase[J]. Chinese Chemical Letters, 2003, 14(6): 619-622.
Citation: Jun YAO, Yi LIU, Tian Zhi WANG, Jian Ben LIU, Peng LIU, Zi Niu YU. Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase[J]. Chinese Chemical Letters, 2003, 14(6): 619-622.
Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase
摘要:
Through the method of initial heat release rate, the kinetic property of tyrosine oxidationcatalyzed by tyrosinase from Pseudomonas maltophilia was investigated using a LKB-2107 batchmicrocalorimeter. Tyrosine was catalyzed and oxidized into L-dopa, then into melanin catalyzed bytyrosinase. We found that the tyrosinase reaction obeyed the Michaelis-Menten kinetics, and at298.15K and pH 7.0, the initial exothermic rate (Ω0) are in the range of0.1567~0.5704 mJ·s-1, the maximum exothermic rate (Ωmax) are in 0.4152~0.8143mol·L-1, and mean value of the Michaelisconstant (Km) is 2.199±0.105×104 mol·L-1.
English
Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase
Abstract:
Through the method of initial heat release rate, the kinetic property of tyrosine oxidationcatalyzed by tyrosinase from Pseudomonas maltophilia was investigated using a LKB-2107 batchmicrocalorimeter. Tyrosine was catalyzed and oxidized into L-dopa, then into melanin catalyzed bytyrosinase. We found that the tyrosinase reaction obeyed the Michaelis-Menten kinetics, and at298.15K and pH 7.0, the initial exothermic rate (Ω0) are in the range of0.1567~0.5704 mJ·s-1, the maximum exothermic rate (Ωmax) are in 0.4152~0.8143mol·L-1, and mean value of the Michaelisconstant (Km) is 2.199±0.105×104 mol·L-1.
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Key words:
- Tyrosine
- / tyrosinase-catalyzed oxidation reaction
- / microcalorimetry
- / thermokinetics
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