Citation:
WANG Sen, LI Bo-Wen, JIANG Long-Guang, WANG Shi-Hua, ZHANG Yan-Feng, WANG Yu. Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase (NDK) from Aspergillus Flavus[J]. Chinese Journal of Structural Chemistry,
2016, 35(11): 1708-1713.
doi:
10.14102/j.cnki.0254-5861.2011-1278
Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase (NDK) from Aspergillus Flavus
摘要:
Aspergillus flavus causes serious disease on important agriculture crops, and its secondary metabolic products-aflatoxins are most potent toxin and carcinogen for animal and human. Structural and functional studies of A. flavus proteins may provide insights into the identification of potential therapeutic targets and prevention of damage caused by A. flavus. Here, we report the expression, purification, crystallization and preliminary crystallographic analysis of NDK protein from A. flavus. The NDK protein was expressed in E. coli and purified by using a series of chromatographic methods to >98% purity. The recombinant protein was crystallized and the crystals diffracted to 2.4 Å resolution. The crystal of NDK is in space group of C121 with a=190.84, b=169.47, c=146.94 Å. Preliminary data analysis indicated that the NDK molecule assembles into a multimer in the asymmetric unit.
English
Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase (NDK) from Aspergillus Flavus
Abstract:
Aspergillus flavus causes serious disease on important agriculture crops, and its secondary metabolic products-aflatoxins are most potent toxin and carcinogen for animal and human. Structural and functional studies of A. flavus proteins may provide insights into the identification of potential therapeutic targets and prevention of damage caused by A. flavus. Here, we report the expression, purification, crystallization and preliminary crystallographic analysis of NDK protein from A. flavus. The NDK protein was expressed in E. coli and purified by using a series of chromatographic methods to >98% purity. The recombinant protein was crystallized and the crystals diffracted to 2.4 Å resolution. The crystal of NDK is in space group of C121 with a=190.84, b=169.47, c=146.94 Å. Preliminary data analysis indicated that the NDK molecule assembles into a multimer in the asymmetric unit.
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Key words:
- Aspergillus flavus
- / nucleoside diphosphate kinase
- / expression
- / purification
- / crystallization
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