引用本文:
Pin YANG, Tian Xin WEI, Chun Ying, Jin Xi WANG, Da Pin FAN. A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator[J]. Chinese Chemical Letters,
1996, 7(9): 820-822.
Citation: Pin YANG, Tian Xin WEI, Chun Ying, Jin Xi WANG, Da Pin FAN. A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator[J]. Chinese Chemical Letters, 1996, 7(9): 820-822.
Citation: Pin YANG, Tian Xin WEI, Chun Ying, Jin Xi WANG, Da Pin FAN. A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator[J]. Chinese Chemical Letters, 1996, 7(9): 820-822.
A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator
摘要:
Fura-2 was first used to monitor intracellular RE3+. The characterizations of RE3+-Fura-2 interactions in solutions simulating intracellular ionic composition show that La3+ and Y3+ both form 1:1 RE3+-fura-2 complexes. The apparent dissociation constants are 1.61×10-7M, and 4.04×10-7M, respectively. The mouse myeloma (SP2/0) cells exposed to free RE3+ or free RE3+ and some small molecules in body were studied by single cell cation measurement system. The results support the assumption that RE3+ ions cannot enter the cytoplasm of living cells, and the small molecules used in our experiments couldn't improve the permeability of RE3+ ions.
English
A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator
Abstract:
Fura-2 was first used to monitor intracellular RE3+. The characterizations of RE3+-Fura-2 interactions in solutions simulating intracellular ionic composition show that La3+ and Y3+ both form 1:1 RE3+-fura-2 complexes. The apparent dissociation constants are 1.61×10-7M, and 4.04×10-7M, respectively. The mouse myeloma (SP2/0) cells exposed to free RE3+ or free RE3+ and some small molecules in body were studied by single cell cation measurement system. The results support the assumption that RE3+ ions cannot enter the cytoplasm of living cells, and the small molecules used in our experiments couldn't improve the permeability of RE3+ ions.
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